Bone marrow stromal cells can promote the neurogenesis in subventricular zone in the rat with focal cerebral ischemia
Authors
Abstract:
Introdution: Stroke is one of the most common diseases caused by occlusion or rupture of blood vessels in brain. It brings heavily loads for families and societies. Although some new strategies including treatment of tissue plasminogen activator have been applied in the clinic, these methods do not have perfect effect. Accordingly, more effective therapeutic strategies need to be developed. This study was conducted to investigate the action of bone marrow stromal cells (BMSC) on the neural stem cells in the subventricular zone of the rat after focal cerebral ischemia. Methods: The rats were induced to permanent focal cerebral ischemia models with middle cerebral artery occlusion (MCA0). Test groups consisted of three groups: MCAO alone, intravenous infusion of 1 ml PBS at 24 hours after MCAO, and intravenous infusion of 2×106 BMSCs 24 hours after MCAO. Then, the groups were divided to investigate at 7 and 14 days after MACO. Neurological functions were detected to use Zausinger evaluation meanwhile, 5-bromodeoxyuridine was injected to label the proliferating cells in the subventricular zone, and double-immunofluoroscent technologies were used to identify the cell type. Results: Neurological functional scores of BMSCs-treated group were higher than other two groups (p<0.05) at 7 and 14 days after MACO. BrdU-positive cells in SVZ of ipsilateral ischemia of BMSCs-treated group were more than two controls (p<0.05) at 14 days after MCAO double-immunofluorescence label demonstrated that BrdU-positive cells co-located with markers of neuron and astrocyte. Discussion: BMSCs can promote the neurological function of the rats with permanent focal cerebral ischemia, which may associate with the neurogenesis in the subventricular zone.
similar resources
bone marrow stromal cells can promote the neurogenesis in subventricular zone in the rat with focal cerebral ischemia
introdution: stroke is one of the most common diseases caused by occlusion or rupture of blood vessels in brain. it brings heavily loads for families and societies. although some new strategies including treatment of tissue plasminogen activator have been applied in the clinic, these methods do not have perfect effect. accordingly, more effective therapeutic strategies need to be developed. thi...
full textBone Marrow Stromal Cells Can Promote The Neurogenesis in Subventricular Zone in The Rat With Focal Cerebral Ischemia
full text
Neurogenesis in dentate subgranular zone and rostral subventricular zone after focal cerebral ischemia in the rat.
Because neurogenesis persists in the adult mammalian brain and can be regulated by physiological and pathological events, we investigated its possible involvement in the brain's response to focal cerebral ischemia. Ischemia was induced by occlusion of the middle cerebral artery in the rat for 90 min, and proliferating cells were labeled with 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdUrd) ove...
full textThe Effect of TGF-alpha on Neurogenesis in Subventricular Zone of Rat Brain after Ischemia-Reperfusion
Introduction: Stroke is the third important reason of death in adults and an important cause of adult disability. Previous studies suggest that TGF-alpha can induce neurogenesis after stroke. Here in, we studied neurogenesis effects of the TGF-alpha on subventricular zone following ischemia-reperfusion. Male wistar rats (250-300 g) were divided into ischemia and treatment groups. After inductio...
full textToxicity Study Of gelatin-chitosan Films with Bone Marrow Stromal Cells Cultured in Rat
Background :Gelatin and chitosan are known as biodegradable and biocompatible biopolymers.These biopolymers have recently receivedincreasingly more attention for tissueengineering.The aim of this study was to evaluate the toxicity effects of gelatin-chitosan film on bone marrow stromal cell(BMSCs) culture in rat. Materials & Methods: First, gel...
full textSodium orthovanadate enhances proliferation of progenitor cells in the adult rat subventricular zone after focal cerebral ischemia.
Neuronal progenitor cells able to produce new neuron and glia persist in the adult central nervous system (CNS). Their proliferation is up-regulated by growth factors or cytokines under some pathological conditions, including ischemia. Because sodium orthovanadate (SOV), a protein tyrosine phosphatase inhibitor, can up-regulate tyrosine kinase-linked growth factor receptor signaling via the inh...
full textMy Resources
Journal title
volume 1 issue 2
pages 15- 19
publication date 2010-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023